Tuesday, January 10, 2017

Virtual Memory T cells

New article brings into question how T cell memory should be defined.

We conducted this research to answer a fundamental question in immunology- Where do antigen inexperienced CD4+ T cells reside? 

Currently, it is widely accepted that central and effector memory CD4+ T cells originate from naïve T cells after they have encountered their cognate antigen in the setting of appropriate co-stimulation. However, if this were true the diversity of T cell receptor (TCR) sequences within the naïve T cell compartment should be far greater than that of the memory T cell compartment, which is not supported by TCR sequencing data. In fact the vast majority of T cells with a "memory" phenotype do not appear to have undergone a strong clonal expansion event.

 In our study we demonstrate that aged mice with far fewer naïve T cells, respond to the model antigen, hen eggwhite lysozyme (HEL), by utilizing the same TCR sequence as their younger counterparts. CD4+ T cell repertoire analysis of highly purified T cell populations from HEL-naive animals revealed that the HEL-specific clones, within these mice, displayed effector and central “memory” cell surface phenotypes even though they had never encountered the HEL antigen. Although cross reactivity to some other foreign antigen exposure could have explained these results, the effector and central “memory” HEL-specific T cells that were detected resided at an extremely low frequencies, identical to their frequency within the naive T cell compartment. Thus, any prior cross recognition event was not strong enough to have induced an expansion of the HEL-specific T cells, at least not one that could be detected above the background level that was seen in the naive T cell compartment. In our experiments the HEL-inexperienced CD4+ T cells were found to reside within the naïve, regulatory, central memory, and effector memory T cell populations at similar frequencies.  The vast majority of the other CD4+ T cells within the regulatory and memory populations were also unexpanded.

 These findings support a new paradigm for CD4+ T cell maturation in which a specific clone can undergo a differentiation process to exhibit a “memory” or regulatory phenotype without having undergone a clonal expansion event. They also demonstrate that a foreign-specific T cell is just as likely to reside within the regulatory T cell compartment as it would the naïve compartment, arguing against the specificity of the regulatory T cell compartment being skewed towards self-reactive T cell clones. Finally, we demonstrate that the same set of foreign T cell clones are repetitively generated throughout adulthood.

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  23. HOW I WAS ABLE TO FLUSH OUT THE HERPES VIRUS WITH THE HELP OF DR. KHAM NATURAL PRODUCT, HIS EMAIL dr.khamcaregiver@gmail.com. I was diagnosed with Herpes a year ago. It’s the worst thing that’s ever happened to me and  I feel like my life is over. Living with constant pain is something no one should have to go through, Herpes has also had a huge impact on my sleep . Since being diagnosed I’ve fallen so far into depression I see no way out because I have been to different hospitals. I’m taking a high dose of antidepressants, as well as sedatives and sleeping-pills. I don’t know how I’ll ever accept that this is my life now…I keep asking why me because now changes? But I guess reading your testimony really made me feel happy because i believe what i had from people out there who you also help to cure the herpes virus, and i believe so much with your herbal medicine i am going to be out of this bad situation like others you also help before, what we i have done if not for the herbal medicine that i requested for that was able to flush out the bad virus that was making me go crazy. people out there will thank this man called DR. KHAM, I will advise you to email him directly if you have diseases like herpes etc. dr.khamcaregiver@gmail.com or whatsapp him +2348159922297, you can also visit his website to know more about him at  https://herbal-dr-kham.jimdosite.com/

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  24. I suffered for four years. I have visited CVS Pharmacy in CA for treatment but no improvement. I met Dr.OMONGE online while I was searching for a herpes cure. I read a testimony about his work. I give a try that now becomes a testimony in my life. I contacted Dr.OMONGBE WhatsApp +2348050407265 or Email: dromongbenaturalremedies@gmail.com His website https://dromongbenaturalre.wixsite.com/dromongbenaturalreme

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  25. Health is not just physical health, but also mental, emotional and spiritual well-being. An important part of the healing process for me is to be active in herpes education, support and advocacy. I volunteer in a number of Herpes organizations, and in particular Positive Men, because the impact of Herpes on women is very different from men. I was once a herpes positive, I will forever be grateful to God Almighty and Dr. IYOHA Solution Temple for reaching me when I thought it was all over, Today I am happy with my family living free. After the medical doctor confirmed Herpes status Negative, after 3 weeks on Dr. IYOHA herbs . I have never in my life believed that herpes could be cured by any herbal medicine i first doubted but i have no choice but to trust and believe him and today i am happy to say i am Herpes free, I want to make sure that herpes never happens to anyone else. We can't prevent herpes by punishment, by stigma and discrimination. It's only through building a safe, supportive and caring environment, that positive people still have hope and they should contact Dr. IYOHA via email: Cantact the great herbal doctor through his what-sapp Via: +1 857 228 8347 or call / Text him : +1 407 337 9869 THANKS .

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