Monday, July 23, 2018


Uncovering insights hidden within the psoriasis transcriptome.

Herein we explain how our novel data mining strategies for RNA-Seq datasets lead to new insights into the pathophysiology of psoraisis, an immune-mediated disease involving the skin. This story can also be found in our recently published manuscript in JCI Insight. You can click here to read the entire publication (subscription NOT required).

Introduction

The skin transcriptome is the sum of all messenger RNA molecules expressed in the skin. About a decade ago, next generation DNA sequencing technologies allowed researchers to investigate the skin transcriptome in the setting of psoriasis. This led to the discovery of thousands of genes that were differentially expressed in psoriasis skin; But are all of these genes involved in psoriasis pathophysiology? As is the case with other diseases, knowing the sum of all expressed genes in psoriasis is only a small piece of the puzzle. How to decipher the psoriasis transcriptome has been a challenge that our research team and others are still working on. One strategy to narrow down the thousands of differentially genes to a more reasonable number is to set a threshold as to what represents a meaningful up or down regulation in gene expression. For example, is it meaningful if a gene's expression is increased by only 1.2 fold in the setting of psoriasis?
Meaningfulness is different from significance, which simply means that the observed change is not likely to be due chance. When analyzing DNA sequencing data for significance, p values need to be adjusted for multiple testing, as transcriptome datasets have thousands of genes. Programs used for such tasks take many factors into consideration (number of uniquely mapping reads, total number of genes monitored, variation in read counts etc). However, even when thresholds for significance and meaningfulness are met, investigators can easily be led astray - as data crunching by these classical methods often over or under emphasize the importance of a gene.

The error of meaningfulness thresholds

It is common to set a 2-fold increase or decrease in gene expression as a threshold for meaningfulness. However, is this the best way to identify differentially expressed genes of interest? It depends on the gene, its expression level, and the situation. Lets take for example TNF. During the 1990's, an area of immunology when virtually all cytokines were categorized as belonging to either Th1 or Th2 immune responses, TNF and IFN-g were viewed as the classical Th1 cytokines. This led to the testing of anti-IFN-g and anti-TNF-based therapies in animal models of autoimmunity. In such experiments, anti-TNF therapies showed superior efficacy. In contrast, anti-IFN-g treatments sometimes made autoimmunity paradoxically more severe. Eventually, anti-TNF therapies also proved useful in treating a variety of human autoimmune diseases. With regards to psoriasis, anti-TNF biologic therapies are indeed effective treatments. Although newer psoriasis biologic treatments target different molecules, the prototypic anti-TNF agent, infliximab, is still arguable one of the fastest and most effective medications for psoriasis (in terms of skin clearing at 10 weeks). However, the effectiveness of anti-TNF-based therapies would not have been predicted from TNF gene expression in psoriasis plaques. In comparison to healthy control skin or uninvolved skin, TNF expression within a psoriatic plaque is only marginally increased. In reality, TNF is made by numerous cell types not simply Th1 cells and relatively small changes in its expression have dramatic effects on tissue homeostasis. Thus, TNF blockade has dramatic effects on pathogenic immune responses. Setting a 2-fold change as a threshold for meaningfulness would absolutely miss important genes such as TNF.
For the next example lets consider a hypothetical gene expressed by T cells. If in the setting of psoriasis the expression of this hypothetical gene drops by 30% in T cells, its expression in psoriatic skin will paradoxical not be decreased at all. In fact, its tissue expression will rise more than 2-fold, which may lead some investigators to erroneously conclude that the "up-regulated" gene is involved in the pathophysiology of psoriasis. In this example, the hypothetical gene is decreased in T cells but increased in psoriasis plaques. The increased expression is a result of the simple fact that psoriatic plaques have more T cells not that the expression of the gene is otherwise increased. It is likely that the vast majority of the upregulated genes in psoriasis are a mere consequence of the infiltrating immune cells or proliferation of existing cells within the skin. These are just some examples of why deciphering transcriptomic data is difficult. One method we have chosen to overcome some of these issues is to look at relative gene expression, that is the expression of one gene relative to reference immune genes, especially the genes that encode immune receptors such as the T cell receptor genes. Another strategy is to do single cell sequencing. Both strategies have advantages and disadvantages. For example, the cell preparation techniques used for single cell sequencing will alter the cellular transcriptome.

T cell receptors and T cell repertoire analysis

As just stated, we often use TCR genes as reference genes when characterizing an immune response. However, this strategy has a variety of barriers. TCRs are encoded by productive rearrangements of the alpha beta or gamma delta TCR genes. For the beta and delta chains, the genetic recombination events involve variable (V), diversity (D), joining (J), and constant (C) region exons. This allows for the immune system to express an almost infinite number of immune receptors to project of from invading pathogens and cancer.
Traditionally, TCR genes are sequenced directly from the products of TCR-specific PCR reactions. TCR mining of RNA-Seq datasets is an alternative approach to characterize T cell repertoires, but requires the use of an appropriate bioinformatics alignment pipeline. In our recent JCI Insight manuscript we developed and validated a pipeline to estimate the overall and relative expression of TCR V/J/C segments. Our method has the advantage over traditional TCR repertoire analysis in that it allows investigators to conduct correlative studies between TCR genes and other genes of interest; this is because it detects all TCR-mapping reads in an RNA-Seq dataset. In contrast, bioinformatics tools focused solely on analysis of the TCR CDR3 region cannot accurately estimate TCR gene segment usage and CDR3-mapping only yields a small handful of reads per RNA-Seq dataset, making correlative studies difficult. Also, traditional RNA-Seq analysis pipelines are not aware of TCR rearrangements and may not take into account gene rearrangements, considering them low quality mapping reads.
Our approach allowed us to not only identify novel psoriasis-associated TCR gene segments, but also correlate the transcription of individual alpha beta and gamma delta TCR gene segments with genes of known importance to the pathophysiology of psoriasis. Four independently acquired RNA-Seq sample sets allowed for rigorous statistical validation of our results.
Our manuscript highlights a many exciting findings. For example, by focusing on the expression of individual TCR gene segments relative to all TCR gene segments of the same family, we discovered that TRAJ23 is proportionally over expressed and TRAJ39 is proportionally under expressed in psoriasis (Bonferroni p = 6.19 x 10e-27 and 1.43 x 10e-248, respectively). (TRAJ23 and TRAJ39 are TCR alpha gene segments). We also found that a TCR gamma gene segment is minimally over expressed in psoriasis, TRGV5 (Bonferroni p = 6.03 x 10e-13)). A finding that we were able to validate across 4 different RNA-Seq datasets. The figure below summarizes these results. Please see the full manuscript for more details. This same strategy can be applied to other autoimmune and cancer datasets, and thus carries wide applications. Importantly, our results differ from the alpha beta gene segments previously reported to be disproportionately overrepresented in psoriasis (1-3). These highly conflicting results may be due to the exceedingly small sample sizes used in the prior studies, which for the most part lacked summary statistics to evaluate the significance of the psoriasis-associated TCR genes.

T cells, IL-17A, and psoriasis

Since the demonstrated efficacy of cyclosporine in psoriasis (4), alpha beta T cells have been thought to be central to psoriasis pathophysiology, and recent clinical evidence points to the Th17 subtype as the driver of disease (5), as is evident by the success of IL-17/IL-23-targeted therapies (6-9). However, many groups are pursuing other sources of IL-17 as important mediators of the psoriatic phenotype. For example, some investigators have posited that in psoriasis, gamma delta T cells are expanded disproportionately more than alpha beta T cells, and that the former is the dominant source of IL-17 (10-12).
To address the debate of alpha beta versus gamma delta T cells head on we compared the relative frequencies of the corresponding alpha, beta, gamma, delta gene segments in psoriatic and normal skin (surprisingly nobody has done this before). By looking at relative gene expression, we found no evidence for a differential expansion of gamma delta T cells over alpha beta T cells. In fact, gamma delta TCR gene segments were only marginally over expressed in the setting of psoriasis. We next sought to address the source of IL-17 by plotting the expression of individual TCR gene segments against IL17A expression. Again, we found no evidence that gamma delta TCR gene segments correlated with IL17A expression. Interestingly, TRGV5 expression did correlate with IL36. In contrast, TRAJ23 expression correlated well with IL17A. These results were then validated in four independently collected RNA-Seq datasets. From these results we speculate that TRAJ23-expressing cells (abundant in psoriasis) are a source of IL-17 and TRGV5-expressing T cells possibly expand in response to IL-36, a psoriasis-associated cytokine. These results are summarized in the figure below. For more details of this study and to see our other exciting discoveries please see the published manuscript by clicking here.







1.  Chang YT, Liu HN, Shiao YM, Lin MW, Lee DD, Liu MT, et al. A study of PSORS1C1 gene polymorphisms in Chinese patients with psoriasis. The British journal of dermatology. 2005;153(1):90-6.
2.  Menssen A, Trommler P, Vollmer S, Schendel D, Albert E, Gurtler L, et al. Evidence for an antigen-specific cellular immune response in skin lesions of patients with psoriasis vulgaris. Journal of immunology. 1995;155(8):4078-83.
3.  Vollmer S, Menssen A, and Prinz JC. Dominant lesional T cell receptor rearrangements persist in relapsing psoriasis but are absent from nonlesional skin: evidence for a stable antigen-specific pathogenic T cell response in psoriasis vulgaris. The Journal of investigative dermatology. 2001;117(5):1296-301.
4.    Ellis CN, Fradin MS, Messana JM, Brown MD, Siegel MT, Hartley AH, et al. Cyclosporine for plaque-type psoriasis. Results of a multidose, double-blind trial. N Engl J Med. 1991;324(5):277-84.
5.    Thaci D, Blauvelt A, Reich K, Tsai TF, Vanaclocha F, Kingo K, et al. Secukinumab is superior to ustekinumab in clearing skin of subjects with moderate to severe plaque psoriasis: CLEAR, a randomized controlled trial. J Am Acad Dermatol. 2015;73(3):400-9.
6.    Langley RG, Elewski BE, Lebwohl M, Reich K, Griffiths CE, Papp K, et al. Secukinumab in plaque psoriasis--results of two phase 3 trials. N Engl J Med. 2014;371(4):326-38.
7.    McInnes IB, Mease PJ, Kirkham B, Kavanaugh A, Ritchlin CT, Rahman P, et al. Secukinumab, a human anti-interleukin-17A monoclonal antibody, in patients with psoriatic arthritis (FUTURE 2): a randomised, double-blind, placebo-controlled, phase 3 trial. Lancet. 2015;386(9999):1137-46.
8.    Papp KA, Leonardi C, Menter A, Ortonne JP, Krueger JG, Kricorian G, et al. Brodalumab, an anti-interleukin-17-receptor antibody for psoriasis. N Engl J Med. 2012;366(13):1181-9.
9.    Gordon KB, Blauvelt A, Papp KA, Langley RG, Luger T, Ohtsuki M, et al. Phase 3 Trials of Ixekizumab in Moderate-to-Severe Plaque Psoriasis. N Engl J Med. 2016;375(4):345-56.
10.  Cai Y, Shen X, Ding C, Qi C, Li K, Li X, et al. Pivotal role of dermal IL-17-producing gammadelta T cells in skin inflammation. Immunity. 2011;35(4):596-610.
11.   Yoshiki R, Kabashima K, Honda T, Nakamizo S, Sawada Y, Sugita K, et al. IL-23 from Langerhans cells is required for the development of imiquimod-induced psoriasis-like dermatitis by induction of IL-17A-producing gammadelta T cells. J Invest Dermatol. 2014;134(7):1912-21.
12.  Hartwig T, Pantelyushin S, Croxford AL, Kulig P, and Becher B. Dermal IL-17-producing gammadelta T cells establish long-lived memory in the skin. Eur J Immunol. 2015;45(11):3022-33.

44 comments:

  1. My Profound and Utmost Gratitude to Doctor Razor, I am here to Share my testimony about a doctor who helped me remove a burden from my life. I was diagnosed with HSV 1 on March 17, 2017, i didn't bother seeking for medical aide because i know that the Government And medical Scientists Have been lying to us about herpes not Having a complete Cure, so I was thinking of how to get a solution, To make sure i get this Infection out of my body. I went to visiting a friend of mine who happens to be A family friend as well and she asked why i was looking so sad, i explained to her my problem, she told me that she can help me out, she introduced me to a doctor who uses herbal medication to cure HERPES SIMPLEX VIRUS and gave me his email, as well as his Website : https://drrazorherbalhome.wixsite.com/drrazorherbalhome . So I emailed him. He told me all the things I needed to do and also gave me instructions on How i was to take the medicine For 18 days, which I followed properly. After completion of the Dosage the herbalist Requested that i go for a medical Test And i was amazed after my Test Result came out Negative. If you are also heart broken and also need help, you can also email him at: drrazorherbalhome@gmail.com . Whatsapp/Call Doctor Razor via +2349065420442

    ReplyDelete
    Replies
    1. Hello everyone, my name is Alfreed siang  i am here to say a big thank you to my doctor DR OLU who helped me enlarge my penis.i have never had a happy relationship in my life because of my inability to perform well due to my small penis, due to frustration,i went online in search of solution to ending my predicament and than i came across testimony on how DR OLU has helped them, so i contacted him and he promised to help me with penis enlargement,i doubted at first but i gave him a trial and he sent me the product which i used according to his prescription and in less than a week,i saw changes in my penis and it grow to the size i wanted and since then,i am now a happy man and no lady complains again about my penis.if you also need the services of my doctor,you can also contact him on his email..  drolusolutionhome@gmail.com   or his whataspp is +2348140654426

























      Hello everyone, my name is Alfreed siang  i am here to say a big thank you to my doctor DR OLU who helped me enlarge my penis.i have never had a happy relationship in my life because of my inability to perform well due to my small penis, due to frustration,i went online in search of solution to ending my predicament and than i came across testimony on how DR OLU has helped them, so i contacted him and he promised to help me with penis enlargement,i doubted at first but i gave him a trial and he sent me the product which i used according to his prescription and in less than a week,i saw changes in my penis and it grow to the size i wanted and since then,i am now a happy man and no lady complains again about my penis.if you also need the services of my doctor,you can also contact him on his email..  drolusolutionhome@gmail.com   or his whataspp is +2348140654426

      Delete
    2. I've Used Dr Aziba Product to solve my Penis Growth problem of 3.8 inches to 9.0 Inches in Less than Seven Days.
      Two hours after starting the Product, I began to feel its results. My penis was tingling (but it did not hurt), and little by little it was growing. Three days later, it already measured 6.6 inches. I had to do absolutely nothing because it was completely automatic. Six days later, I had gained one more Eight inch. The result: 8.1 inches! I began to feel more confident as I slept with my Girlfriend, who is always willing to do the dirty. In the end, she told me a lot has changed in me and i now gives her so much orgasms in half an hour. i'm recommending you with similar problem to Get In Contact with Doctor Aziba via Email: Priestazibasolutioncenter@gmail.com & WhatsApp (+234)810-0368-288 For Help!








      I've Used Dr Aziba Product to solve my Penis Growth problem of 3.8 inches to 9.0 Inches in Less than Seven Days.
      Two hours after starting the Product, I began to feel its results. My penis was tingling (but it did not hurt), and little by little it was growing. Three days later, it already measured 6.6 inches. I had to do absolutely nothing because it was completely automatic. Six days later, I had gained one more Eight inch. The result: 8.1 inches! I began to feel more confident as I slept with my Girlfriend, who is always willing to do the dirty. In the end, she told me a lot has changed in me and i now gives her so much orgasms in half an hour. i'm recommending you with similar problem to Get In Contact with Doctor Aziba via Email: Priestazibasolutioncenter@gmail.com & WhatsApp (+234)810-0368-288 For Help!

      Delete
    3. HELLO EVERYONE.Natural herbs have cured so many illness that drugs and injection cant cure. I''ve seen the great importance of natural herbs and the wonderful work they have done in people lives. i read testimonies online how they were cured of herpes, HIV, diabetics, cancer etc by Dr. Sayo herbal medicine, so i decided to contact him because i know nature has the power to heal anything. I was diagnosed with herpes for the past 9 months but Dr.Sayo cured me with his herbs and i referred my aunt and her husband to him immediately because they were both suffering from cancer but they were cured too. Contact Dr. Sayo via email on: sayoherbalhealer@gmail.com Or Whatsapp him on: +2349012175679 You can also visit his FB page: https://www.facebook.com/Sayo-Herbal-Healer-100145798345000/ check his website: sayoherbalhome.com

      Delete
    4. This is another testimony on how Chief Dr Lucky cured my HIV disease. Do you need a cure for your HIV disease? Do you want to be cured from your cancer disease? Or you want to be free from any type of disease. Kindly visit his website https://chiefdrluckyherbaltherapy.wordpress.com/ . He just cured my HIV disease and I’m very grateful to him, he is the only herbalist that can cure you.  
      WhatsApp number : +2348132777335 
      Via Email : chiefdrlucky@gmail.com
      Thank you all for reading,
      God bless"

      Delete
  2. Are you in need of finance? we give out guarantee cash at 3% interest rate. Contact us on any kind of finance now: financialserviceoffer876@gmail.com whatspp Number +918929509036 Dr James Eric

    ReplyDelete
    Replies


    1. Words are empty when spoken. A big thank you to Dr Aziba for using his effective herbal product to reserve my ALS disease. I was diagnosed of ALS disease a year back. My symptoms progressed so very fast that i couldn't do anything. I had tripping, dropping, abnormal fatigue of my leg, slurred speech, muscle cramps and twitches. I was very depressed and had no hope of living. i couldn't resist it than to go deep into research for a cure. On this fateful day, i saw a testimony post concerning a lady who once had ALS disease but was reserved using Dr Aziba Natura Herbal product. At this time, i was skeptical and curious. To cut it short, i visited his WhatsApp on +2348100368288 and i saw so many review which motivated me to make my orders. I received the product and used it as instructed for two weeks. Omg, during the second week of usage, all my symptoms disappeared, today makes it 3 months after usage and i can boldly tell to the public that i have been reserved from ALS disease for 3 years span and after which i will make another orders again after 3 years.. This is more than a miracle, i just can't believe my life is back again. last 2 months i resumed work and i can now do all activities myself without anyone's assistance. You that is out there searching for solution, i have bring good news to you kindly Contact Dr via Email. Priestazibasolutioncenter@gmail.com or reach him directly via WhatsApp +2348100368288 Today marks my birthday. Please lets share this to help others. Thank you Dr Aziba for your Herbal product.



      Delete
  3. NATURAL HERPES CURE.Herpes simplex virus is said to have no cure, just as i was told by my family doctor that herpes can not be cured but can be controlled, Although i was a very stubborn person i never believed in what my doctor told me about herpes having no cure. Am a very honest person who believe in nature and i also believed that there could be cure somewhere in the world, I kept my faith so strong and kept on doing my research till i saw an article shared by a testifier on How a herbal Doctor Razor from west Africa, i Decided to use his herbal medicine, so i Reached out to him and explained my situation to him, he promised to guide me, after 4 days of reaching out to him i received my medication sent to me by Doctor Razor and he instructed me to use it for 18 days, After Completion of his herbal medicine dosage, i went for a medical checkup as i was told by Doctor Razor. I am so happy and extremely excited that I got cured of this Virus. Massive gratitude to doctor Razor. Reach out to him via his clinic email : drrazorherbalhome@gmail.com  On whatsapp/call his cell phone +2349065420442. Together we stand a chance to fight herpes. Let's continue to share this till The whole world Gets to know that there is a herbal cure for herpes.God bless you all.

    ReplyDelete
  4. I was able to get rid of my herpes virus this year, i was diagnosed of herpes virus since late 2015 and i was on my doctor prescription ever since i have the virus, I wasn’t satisfied i needed to get the virus out of my system and get rid of the virus,i was going through a blog to find new informations about the Herpes Simplex Virus i came across a review of a lady who was testify on the  cured that the great herbalist cured is virus and  i have to contert him and see what he told me to do for me to get rid of the cure I contacted the herbal foundation and they guided me on how to purchase the herbal formula. the herbal products las me for 3 weeks which helps me to get rid of the virus totally, for easy communication you can reach Dr Freeman Osas on his WhatSapp via +2347057052206 email. balogospellcaster@gmail.com     

    ReplyDelete
  5. I was able to get rid of my herpes virus this year, i was diagnosed of herpes virus since late 2015 and i was on my doctor prescription ever since i have the virus, I wasn’t satisfied i needed to get the virus out of my system and get rid of the virus,i was going through a blog to find new informations about the Herpes Simplex Virus i came across a review of a lady who was testify on the  cured that the great herbalist cured is virus and  i have to contert him and see what he told me to do for me to get rid of the cure I contacted the herbal foundation and they guided me on how to purchase the herbal formula. the herbal products las me for 3 weeks which helps me to get rid of the virus totally, for easy communication you can reach Dr Freeman Osas on his WhatSapp via +2347057052206 email. balogospellcaster@gmail.com     

    ReplyDelete
  6. Definitely and totally cured of herpes virus by Doctor Razor's Quick cure for Herpes. My Sincere Gratitude to Dr Razor, I was infected with HERPES SIMPLEX VIRUS 2 November 13 2018, I went to many hospitals for cure but there was no solution, I was confused because the medical drugs has become my daily food, but i decided to sort after Natural Herbs. One day I sat beside the pool, Browsing and thinking where I could get a solution to end this predicament. I saw a blog on how Dr Razor cured people with his herbal medicine, i did not believe but i just decided to give him a try, Visit Doctor's Razor Website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome . I contacted him and he prepared the herbs for me which I took, and he instructed me to go for a check up, after the test i was confirmed with herpes negative, i am so happy. If you have any problem or you are also infected with any disease, kindly contact him now with his Email: drrazorherbalhome@gmail.com or Whatsapp/call +2349065420442 . This testimonial serves as an expression of my gratitude.He also have a herbal cure for COLD SORE,SHINGLES,CANCER(Cannabis Oil),HPV,ASTHMA,IMPOTENCE,BARENESS/INFERTILITY ..Contact him on this email: drrazorherbalhome@gmail.com , or Whatsapp/call his cell phone number on +2349065420442

    ReplyDelete
  7. Natural herbs have cured so many illness that drugs and injection can’t cure. I've seen the great importance of natural herbs and the wonderful work they have done in people's lives. i read people's testimonies online on how they were cured of HERPES, HIV, diabetics etc by Dr Edes herbal medicine, so i decided to contact the doctor because i know nature has the power to heal anything. I was diagnosed with Herpes for the past 3 years but Dr Edes cured me with his herbs and i referred my aunt and her husband to him immediately because they were both suffering from Herpes but to God be the glory, they were cured too .I know is hard to believe but am a living testimony. There is no harm trying herbs, Thanks. Write him on WhatsApp on +2348151937428. @dr_edes_remedies deals with
    (1)Alzheimer virus
    (2)Cancer
    (3)HIV
    (4)Herpes
    (5)Genital warts
    (6)ALS
    (7)Virginal infection
    (8)HPV
    (9)Hepatitis
    (10)Asthma
    Email him on dredeshome@gmail.com
    https://dredesherbalhome.weebly.com/product-order.html
    https://www.youtube.com/channel/UCWhBHvtpo772EWAEdsFWmWQ

    ReplyDelete
  8. My ex-husband and I had always managed to stay friendly after our divorce in February 2017. But I always wanted to get back together with him, All it took was a visit to this spell casters website last December, because my dream was to start a new year with my husband, and live happily with him.. This spell caster requested a specific love spell for me and my husband, and I accepted it. And this powerful spell caster began to work his magic. And 48 hours after this spell caster worked for me, my husband called me back for us to be together again, and he was remorseful for all his wrong deeds. My spell is working because guess what: My “husband” is back and we are making preparations on how to go to court and withdraw our divorce papers ASAP. This is nothing short of a miracle. Thank you Dr Emu for your powerful spells. Words are not enough. here is his Email: emutemple@gmail.com or call/text him on his WhatsApp +2347012841542    

    ReplyDelete
  9. Quick Cure I will advise you to contact Dr KHAM AT (dr.khamcaregiver@gmail.com ) where all problems are solved without stress and delay. My family was getting drawn in sickness, we were all affected one after the other and medical prescription was not helping us. Till a friend's doctor directs us to contact Dr KHAM for quick cure and total cleaning. Dr KHAM is a miracle worker. It is never too late with Dr KHAM contact him today with { dr.khamcaregiver@gmail.com  } you can also contact him on Whatsapp +2348159922297, You can Also Visit his website to know more about him at >https://herbal-dr-kham.jimdosite.com/ or visit his Blog at https://drkhamcaregiver.blogspot.com/  
    He also Cure
    1) HIV/AIDs Virus
    2) Hepatitis B and C
    3) Herpes
    4) Cancer
    5) ZIKA VIRUS
    6) COPD
    7) STROKE.
    8) Erectile Dysfunction
    9) Trichomoniasis
    10) Chlamydia
    11) Gonorrhea
    12) HPV
    13) Syphilis        

    ReplyDelete
  10. Hey �� everyone. I just wanna tell y’all that I’ve been cured from herpes virus. It only took me Two weeks to be cured finally from this virus and what I did definitely got me cured completely from my HSV2, I’m so happy ������. When every odd seems to be against me i trusted in the lord. I battled for so long until a friend introduced me to Doctor Kham who was finally able to help me and here i am today healthy and strong. Email this herbalist via dr.khamcaregiver@gmail.com that is his contact details.

    ReplyDelete


  11. Words are empty when spoken. A big thank you to Dr Aziba for using his effective herbal product to reserve my ALS disease. I was diagnosed of ALS disease a year back. My symptoms progressed so very fast that i couldn't do anything. I had tripping, dropping, abnormal fatigue of my leg, slurred speech, muscle cramps and twitches. I was very depressed and had no hope of living. i couldn't resist it than to go deep into research for a cure. On this fateful day, i saw a testimony post concerning a lady who once had ALS disease but was reserved using Dr Aziba Natura Herbal product. At this time, i was skeptical and curious. To cut it short, i visited his WhatsApp on +2348100368288 and i saw so many review which motivated me to make my orders. I received the product and used it as instructed for two weeks. Omg, during the second week of usage, all my symptoms disappeared, today makes it 3 months after usage and i can boldly tell to the public that i have been reserved from ALS disease for 3 years span and after which i will make another orders again after 3 years.. This is more than a miracle, i just can't believe my life is back again. last 2 months i resumed work and i can now do all activities myself without anyone's assistance. You that is out there searching for solution, i have bring good news to you kindly Contact Dr via Email. Priestazibasolutioncenter@gmail.com or reach him directly via WhatsApp +2348100368288 Today marks my birthday. Please lets share this to help others. Thank you Dr Aziba for your Herbal product.



    ReplyDelete
  12. HERBAL DR EMU WHO PREPARE HERBAL MEDICINE TO CURE ALL KINDS OF DISEASES INCLUDING HERPES DISEASE.   
     I have been battling this Herpes disease for almost 3 years now....I tried all possible means to get cure from my Herpes Disease but all to be in vain until i saw a post in a health forum about a herbal Dr Emu who prepare herbal medicine to cure all kind of diseases including Herpes Disease, at first i doubted if it was real but decided to give it a try...when i contact Dr Emu via his email (emutemple@gmail.com)  write him and reply me explain how the process work so after ordering for the medicine I got it within 3/4 working days through DHL Delivery and I took it according to the way Dr Emu instructed, I was so happy after 2 week I took the medicine there was very big change in my health when I was done with the process I go for test, I found out I am negative...Herpes patients should also get in touch with this herbalist Dr Emu to get rid of these Herpes Virus forever his whatsapp number +2347012841542.

    ReplyDelete
  13. My name is Brittney Brian,i am from Arizona, Its a pleasure for me to write this testimony about how i got my Genital Herpes cured 2 months ago. i have been reading so many comments of some people who were cured from various diseases by Dr. Festus, I was hurt and depressed so I was too curious and wanted to try Dr. Festus cuz have been burdened by this virus for many , then i contacted him through his email when i contact him, he assured me 100% that he will heal me, i pleaded with him to help me out which he did, he healed me just as he promised, he sent me his medication and ask me to go for check up after 5 weeks of taking the medication. i agreed with him i took this medication and went for check up, to my greatest surprise my result was negative after the treatment, i am really happy that i am cured and healthy again. I have waited for 5 weeks after I first tested negative just to be very sure i was completely healed before writing this testimony I just got back from the hospital today from getting my blood test result and am still negative, so i guess its time i recommend anyone going through Herpes HSV-1 or HSV-2, HIV, HPV, Hepatitis A&B, Diabetes, Cancer reach him through Email drfestusherbalsolutioncentre@gmail.com OR add on whatsapp +2348073742265.

    ReplyDelete
  14. I have been a herpes carrier for 2 years and I tried every possible means
    of curing but all of no useful until I saw a health promotion on a
    herbalist from Africa who prepares herbal medicines to cure all sorts of
    diseases including cancer and many others sickness, I first doubted It was
    not true but decided to try, when I contacted this herbal physician via his
    email, I purchased herpes herbal medicine and received it within 7 days
    and used it as prescribed, I tested negative within 3 weeks of use, do not
    lose hope to contact him early If you need help, am convinced he can help you too,
    He is also special in curing 1.
    HIV/AIDS 2. HERPES 3. CANCER 4. ALS 5. HEPATITIS B 6. DIABETES 7. HUMAN
    PAPILLOMAVIRUS DISEASE(HPV) 8. ALZHEIMER 9. LUPUS (Lupus Vulgaris or Lupus Eratosthenes)ETC,,,,,, You can also visit his website:
    http://drchalaherbalhome.godaddysites.com
     email him on dr.chalaherbalhome@gmail.com

    ReplyDelete
  15. I've Used Dr Aziba Product to solve my Penis Growth problem of 3.8 inches to 9.0 Inches in Less than Seven Days.
    Two hours after starting the Product, I began to feel its results. My penis was tingling (but it did not hurt), and little by little it was growing. Three days later, it already measured 6.6 inches. I had to do absolutely nothing because it was completely automatic. Six days later, I had gained one more Eight inch. The result: 8.1 inches! I began to feel more confident as I slept with my Girlfriend, who is always willing to do the dirty. In the end, she told me a lot has changed in me and i now gives her so much orgasms in half an hour. i'm recommending you with similar problem to Get In Contact with Doctor Aziba via Email: Priestazibasolutioncenter@gmail.com & WhatsApp (+234)810-0368-288 For Help!

    ReplyDelete
  16. I've Used Dr Aziba Product to solve my Penis Growth problem of 3.8 inches to 9.0 Inches in Less than Seven Days.
    Two hours after starting the Product, I began to feel its results. My penis was tingling (but it did not hurt), and little by little it was growing. Three days later, it already measured 6.6 inches. I had to do absolutely nothing because it was completely automatic. Six days later, I had gained one more Eight inch. The result: 8.1 inches! I began to feel more confident as I slept with my Girlfriend, who is always willing to do the dirty. In the end, she told me a lot has changed in me and i now gives her so much orgasms in half an hour. i'm recommending you with similar problem to Get In Contact with Doctor Aziba via Email: Priestazibasolutioncenter@gmail.com & WhatsApp (+234)810-0368-288 For Help!

    ReplyDelete
  17. Hello Am From The UK , I want to write a little testimony about the good work of doctor Oyagu who cured me from Hsv 1 and 2 for just 2 weeks with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this doc Oyagu email on a blog of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through email address oyaguherbalhome@gmail.com or text him on what's app, phone number +2348101755322 or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome Dr Oyagu also has remedy to others disease like COLD SORES,HIV/AIDS,DIABETES.CANCER,HIGH BLOOD PRESSURE AND MANY MORE. I oblige everyone to contact this powerful herbalist Dr Oyagu and be free from suffering.

    ReplyDelete
  18. HOW I WAS ABLE TO FLUSH OUT THE HERPES VIRUS WITH THE HELP OF DR. KHAM NATURAL PRODUCT, HIS EMAIL dr.khamcaregiver@gmail.com. I was diagnosed with Herpes a year ago. It’s the worst thing that’s ever happened to me and  I feel like my life is over. Living with constant pain is something no one should have to go through, Herpes has also had a huge impact on my sleep . Since being diagnosed I’ve fallen so far into depression I see no way out because I have been to different hospitals. I’m taking a high dose of antidepressants, as well as sedatives and sleeping-pills. I don’t know how I’ll ever accept that this is my life now…I keep asking why me because now changes? But I guess reading your testimony really made me feel happy because i believe what i had from people out there who you also help to cure the herpes virus, and i believe so much with your herbal medicine i am going to be out of this bad situation like others you also help before, what we i have done if not for the herbal medicine that i requested for that was able to flush out the bad virus that was making me go crazy. people out there will thank this man called DR. KHAM, I will advise you to email him directly if you have diseases like herpes etc. dr.khamcaregiver@gmail.com or whatsapp him +2348159922297, you can also visit his website to know more about him at  https://herbal-dr-kham.jimdosite.com/

    ReplyDelete
  19. Definitely and totally cured of herpes virus by Doctor Razor's Quick cure for Herpes. My Sincere Gratitude to Dr Razor, I was infected with HERPES SIMPLEX VIRUS 2 November 13 2018, I went to many hospitals for cure but there was no solution, I was confused because the medical drugs has become my daily food, but i decided to sort after Natural Herbs. One day I sat beside the pool, Browsing and thinking where I could get a solution to end this predicament. I saw a blog on how Dr Razor cured people with his herbal medicine, i did not believe but i just decided to give him a try, Visit Doctor's Razor Website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome . I contacted him and he prepared the herbs for me which I took, and he instructed me to go for a check up, after the test i was confirmed with herpes negative, i am so happy. If you have any problem or you are also infected with any disease, kindly contact him now with his Email: drrazorherbalhome@gmail.com or Whatsapp/call +2349065420442This testimonial serves as an expression of my gratitude.
    He also have a herbal cure for COLD SORE,
    SHINGLES,
    CANCER(Cannabis Oil),
    HPV,
    ASTHMA,
    IMPOTENCE,
    BARENESS/INFERTILITY ..
    Contact him on this email: drrazorherbalhome@gmail.com , or Whatsapp/call his cell phone number on +2349065420442

    ReplyDelete
  20. This is very helpful with no side effect...Before I began Doctor Aziba natural penis enlargement Product I measured 4 Inches in erect length, and 3 inches in erect girth. While flaccid I measured 3.4 Inches long. I began using his product, slightly skeptical I admit, and just 2 weeks later I was very happy with my progress. I measured 7.5 inches erect length and 6.5 inches erect girth, and 5.5 inches flaccid length. I was actually quite shocked at the huge gains I’d had, and continued using the product regularly, sticking to the usage schedule rigorously. After about 2-3 weeks I am 8.2 Inches in erect length and 8.1 Inches in erect girth. I am also 7.2 fnches long while flaccid. This has had a huge impact on my life, not only has my sex life improved greatly, I feel much more confident and proud. Dr Aziba Natural Penis Enlargement works! and you can as well reach out to him on any of these cure for ALS, HPV, HSV, HIV, HERPES, Virginal Infection, Heart Burn, Cancer etc... You can Get in Contact with his via Email: (Priestazibasolutioncenter@gmail.com ) or WhatsApp him via contact +2348100368288 His the solution you've been searching !








    This is very helpful with no side effect...Before I began Doctor Aziba natural penis enlargement Product I measured 4 Inches in erect length, and 3 inches in erect girth. While flaccid I measured 3.4 Inches long. I began using his product, slightly skeptical I admit, and just 2 weeks later I was very happy with my progress. I measured 7.5 inches erect length and 6.5 inches erect girth, and 5.5 inches flaccid length. I was actually quite shocked at the huge gains I’d had, and continued using the product regularly, sticking to the usage schedule rigorously. After about 2-3 weeks I am 8.2 Inches in erect length and 8.1 Inches in erect girth. I am also 7.2 fnches long while flaccid. This has had a huge impact on my life, not only has my sex life improved greatly, I feel much more confident and proud. Dr Aziba Natural Penis Enlargement works! and you can as well reach out to him on any of these cure for ALS, HPV, HSV, HIV, HERPES, Virginal Infection, Heart Burn, Cancer etc... You can Get in Contact with his via Email: (Priestazibasolutioncenter@gmail.com ) or WhatsApp him via contact +2348100368288 His the solution you've been searching !

    ReplyDelete
  21. HOW I GOT CURED FROM HERPES VIRUS I was diagnosed of HERPES virus and i have tried all I can to get cured but all to no avail,,until I saw a post in a health forum about a herbalist man who prepare herbal medications to cure all kind of diseases including HERPES virus,at first I doubted if it was real but decided to give it a try,when I contacted this herbalist via his email and he prepared a HERPES herbal cure and sent it to me via UPS delivery company service, when I received this herbal medicine, he gave me step by direction on how to apply it,after applying the way I was instructed,I was totally cured of this deadly disease called HERPES, all thanks to Dr OKONKWO.Email okonkwosolutionplace@gmail.com this great herbal doctor via Whatsapp him on +1561 240 3570
    text him on +1 561 240 3570 HE CAN ALSO HELPS WITH SOME OTHER ISSUES SUCH AS

    HIGH BLOOD PREAURE
    FIBROID DISEASES
    LOW SPERM COUNT.
    WEAK ERECTION.
    {1}HIV/AIDS
    {2}DIABETES
    {3}EPILEPSY
    {4} BLOOD CANCER
    {5} HPV
    {6} ALS
    {7} HEPATITIS
    {8}LOVE SPELL
    {9} SICKLE AND ANAEMIA..
    Email; okonkwosolutionplace@gmail.com
    Whatsapp him on +1561 240 3570
    text him on +1 561 240 3570
    https://fb.me/curefordeadlydideases

    ReplyDelete
  22. Hello everybody
    i don't just know the reason why some people is finding it difficult to believe that there is a cure for herpes, i have been suffering from herpes since last three years with my boyfriend but today i am happy that am cure from it with the herbal medicine of Dr eromonsele the great healer,i was browsing the internet searching for help when i came across a testimony shared by someone on how Dr eromonsele cure his herpes i was so much in need of getting his treatment but after all Dr eromonsele brought a smile to my face with his herbal medicine. i am so much happy today that we have someone like this great healer out there, so my people out there kindly contact this great healer on his email address: dr.eromonsele@gmail.com please sir keep your good work cause there are people out there who is in need of your healing medicine.once more contact him now: dr.eromonsele@gmail.com you can call him or whatsApp his number +2349079308479 

    ReplyDelete
  23. Contact Dr DADA for penis enlargement pills and premature ejaculation and week erection,disfution ,diabetes, Cancer, heart problem, breast enlargement and hip enlargement. Dr DADA  help me with penis enlargement pills so if you are in the same position like me contact him ( drdadaspellhome7@gmail.com ) OR whatsapp him on +2349023126215

    ReplyDelete
  24. Am very glad to share this testimony with everyone for the marvelous work Dr DADA has done for my life, 6months ago i was diagnosed with herpes virus and ever since then i have been very unhappy, i was so down broken everyday, until one day when i came across a shocking testimony about how Dr DADA cured someone. of his herpes virus, without wasting much time i contacted him immediately on his email address: < drdadaspellhome7@gmail.com and after i explain myself to him about how terrible i have been, and he assure me that he will help me to cure my herpes virus,after he has prepared the herbal medicine, he sent it to me and when i have received it and started using it i was totally cure within 2weeks, i am forever grateful to Dr DADA for helping me out with his herpes prescription that cured my virus. contact his email addreSS (drdadaspellhome7@gmail.com or you can call or whatsApp his Mobile number:2349023126215 

    ReplyDelete
  25. Definitely and totally cured of herpes virus by Doctor Razor's Quick cure for Herpes. My Sincere Gratitude to Dr Razor, I was infected with HERPES SIMPLEX VIRUS 2 November 13 2018, I went to many hospitals for cure but there was no solution, I was confused because the medical drugs has become my daily food, but i decided to sort after Natural Herbs. One day I sat beside the pool, Browsing and thinking where I could get a solution to end this predicament. I saw a blog on how Dr Razor cured people with his herbal medicine, i did not believe but i just decided to give him a try, Visit Doctor's Razor Website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome . I contacted him and he prepared the herbs for me which I took, and he instructed me to go for a check up, after the test i was confirmed with herpes negative, i am so happy. If you have any problem or you are also infected with any disease, kindly contact him now with his Email: drrazorherbalhome@gmail.com or Whatsapp/call +2349065420442This testimonial serves as an expression of my gratitude.
    He also have a herbal cure for COLD SORE,
    SHINGLES,
    CANCER(Cannabis Oil),
    HPV,
    ASTHMA,
    IMPOTENCE,
    BARENESS/INFERTILITY ..
    Contact him on this email: drrazorherbalhome@gmail.com , or Whatsapp/call his cell phone number on +2349065420442

    ReplyDelete
  26. Definitely and totally cured of herpes virus by Doctor Razor's Quick cure for Herpes. My Sincere Gratitude to Dr Razor, I was infected with HERPES SIMPLEX VIRUS 2 November 13 2018, I went to many hospitals for cure but there was no solution, I was confused because the medical drugs has become my daily food, but i decided to sort after Natural Herbs. One day I sat beside the pool, Browsing and thinking where I could get a solution to end this predicament. I saw a blog on how Dr Razor cured people with his herbal medicine, i did not believe but i just decided to give him a try, Visit Doctor's Razor Website : https://herbalistrazorherb.wixsite.com/drrazorherbalhome . I contacted him and he prepared the herbs for me which I took, and he instructed me to go for a check up, after the test i was confirmed with herpes negative, i am so happy. If you have any problem or you are also infected with any disease, kindly contact him now with his Email: drrazorherbalhome@gmail.com or Whatsapp/call +2349065420442This testimonial serves as an expression of my gratitude.
    He also have a herbal cure for COLD SORE,
    SHINGLES,
    CANCER(Cannabis Oil),
    HPV,
    ASTHMA,
    IMPOTENCE,
    BARENESS/INFERTILITY ..
    Contact him on this email: drrazorherbalhome@gmail.com , or Whatsapp/call his cell phone number on +2349065420442

    ReplyDelete
  27. HELLO EVERYONE.Natural herbs have cured so many illness that drugs and injection cant cure. I''ve seen the great importance of natural herbs and the wonderful work they have done in people lives. i read testimonies online how they were cured of herpes, HIV, diabetics, cancer etc by Dr. Sayo herbal medicine, so i decided to contact him because i know nature has the power to heal anything. I was diagnosed with herpes for the past 9 months but Dr.Sayo cured me with his herbs and i referred my aunt and her husband to him immediately because they were both suffering from cancer but they were cured too. Contact Dr. Sayo via email on: sayoherbalhealer@gmail.com Or Whatsapp him on: +2349012175679 You can also visit his FB page: https://www.facebook.com/Sayo-Herbal-Healer-100145798345000/ check his website: sayoherbalhome.com

    ReplyDelete
  28. I'm recommending Dr. Kham to everyone who has herpes simplex virus to get the cure from him. I was diagnose of herpes virus in 2018 and i have been searching and asking questions to see if i could get something to cure the disease because i did not believe what the doctors say that there is  no cure to it, I came across a comment on a blog and the person was saying how she was cured from herpes virus  after using Dr. Kham herbal medicine. I quickly contact Dr. Kham and explain my problem to him and he said that i should worry no more that i should be glad for contacting him for help before he prepare the herbal medicine and send it to me through UPS and gave me instructions on how to use it and he assured me  to go for checkup after taking the dosage for two weeks which i did and  after two weeks of taken the herbal medicine and my result was NEGATIVE. I waited another month and I went for another test in a different hospital and the result was still NEGATIVE and my doctor told me that I am completely free from herpes virus. so i came out here to tell those people that say there is no cure for herpes virus that they should stop saying it and they should contact Dr. Kham and see what will happen to them in two weeks time and i believe that you will also come only  here to testify about his good work also, Am so happy and grateful to Dr. Kham for what he has done for me and i will continue to share this for people out there to know that there is cure for herpes. You can contact Dr. Kham on email and WhatsApp to get the cure from him. Email: dr.khamcaregiver@gmail.com and WhatsApp +2348159922297 you can also visit his website to know more about him at  https://drkhamherbalhealingcenter.wordpress.com/  or https://drkhamcaregiver.wixsite.com/drkhamcaregiverherba 

    ReplyDelete
  29. This comment has been removed by the author.

    ReplyDelete
  30. This is really serious, who will believe that a herb can cure herpes, i never believe that this will work, i have spend a lot getting drugs from the hospital to keep me healthy, it got to a time that i was waiting for death to come because i was broke, one day i hard about this great man called Dr. Kham who is well know for Herpes, HIV and Cancer cure, i decided to email him I didn't believe him that much, I just wanted to give him a try, he replied my mail and Needed some Information about me, then I sent them to him, he prepared a herbal medicine (CURE) and sent it through Online Courier Service for delivery, he gave my details to the Courier Office. they told me that 3-5 days I will receive the package and after receiving it, i took the medicine as prescribed by him at the end of the one week, he told me to go to the hospital for a check up, and i went, surprising, after the test the doctor confirm me Herpes simplex virus negative, i thought it was a joke, i went to other hospital and was also negative, thank you for saving my life, I promise I will always testify of your good works. if you are herpes simplex virus patient, OR you are be helping by someone before i will like to advice you to give this man a try and see what will happen to your life don't worry about the money you have being using contact him and I am sure you, you will get cured, WhatsApp him on +2348159922297 or You can as well contact Dr. Kham via email: {dr.khamcaregiver@gmail.com} You can Also Visit his website to know more about him at >https://herbal-dr-kham.jimdosite.com/   or visit his Blog at https://drkhamcaregiver.blogspot.com/  
     DR. KHAM HIS IS ALSO SPECIALIZED ON CURING THE FOLLOWING DISEASES/VIRUS AS FOLLOW,
    (1) CANCER,
    (2) DIABETES,
    (3) HIV AIDS,
    (4) URINARY TRACT INFECTION,
    (5) FIBROID,
    (6) IMPOTENCE,
    (7) BARENESS/INFERTILITY,
    (8) PREGNANCY,
    (9) MONEY SPELL,
    (10) HSV 1,
    (11) ASTHMA,
    (12) HIGH BLOOD PRESSURE,
    (13) HSV 2,
    (14) EX BACK,
    (15) GOOD LUCK IN BUSINESS,
    (16) SUPER POWERS,
    (17) LOW SPERM COUNT
    contact him with his whatsapp Contact (+2348159922297) or {dr.khamcaregiver@gmail.com}
    I can't stop talking about this man called Dr. Kham cause he is God sent to cure humans from different DISEASES with herbal medications, he cured me of  HERPES VIRUS, he will also help you   

    ReplyDelete
  31. This comment has been removed by the author.

    ReplyDelete
  32. Hello Am From The UK , I want to write a little testimony about the good work of doctor Oyagu who cured me from Hsv 1 and 2 for just 2 weeks with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this doc Oyagu email on a blog of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through email address oyaguherbalhome@gmail.com or text him on what's app, phone number +2348101755322 or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome Dr Oyagu also has remedy to others disease like COLD SORES,HIV/AIDS,DIABETES.CANCER,HIGH BLOOD PRESSURE AND MANY MORE. I oblige everyone to contact this powerful herbalist Dr Oyagu and be free from suffering.

    ReplyDelete
  33. To know more about Chief Dr Lucky you can visit his website (https://chiefdrluckyherbaltherapy.wordpress.com/)
     
     A friend that suffered from Herpes and was cure with the help of this great herbal doctor (Chief Lucky) so i decided to contact him for help in getting rid of my families genital herpes virus 1/2 which i did and all i was told to provide was just some useful information and some materials used in preparation of the natural cure and that i did and now i am the happiest person on earth because i am writing this testimony of mine with joy and happiness in my heart to the whole world to know that natural remedy is real because i am a living testimony of Chief Lucky traditional herbal cure and i want you all to contact Chief Lucky via his email: chiefdrlucky@gmail.com or WhatsApp him +2348132777335 am sure he will help you too.

    ReplyDelete
  34. Why taking those drugs and supplements that can't even get rid of the Herpes Virus?. I advise you to contact Herbalist  Kham the caregiver and get cured from your Herpes disease once and forever!, His herbal medicine is the best! trust me, I have no doubt about doctor Kham herbal medicine cause his herbal medicine is so powerful and effective. I’m talking from experience because I was cured from my HSV2 by this great doctor within just two weeks of me using his medication. Forget! about Modern Medicine and Use Herbs, Real medicine comes from the Earth and not from the LAB. For getting rid of your herpes within just two weeks, reach the Herbal doctor on email dr.khamcaregiver@gmail.com or WhatsApp him with his contact info +2348159922297. For more enquiries, you can as well visit the doctor's website by clicking on the link   https://herbal-dr-kham.jimdosite.com or https://dr-kham-herbal-home.webnode.com/   Herbal Doctor KHAM is my lifesaver and i love him so much for getting me cured from my long term genital herpes (HSV2) and i vowed  and promised him that i will keep testifying about him and his good work till my last days on EARTH.  He can also cure: Cancer, Kidney Disease, Diabetes, Fibroid, Staphylococcus, Stroke, Sinuses, Lupus,  Cold Sore, Hepatitis B, Candida Ablicans, Sickle Cell Anemia Disease, Ebola and many more.

    ReplyDelete
  35. Since it needs first financial investment; which is extremely marginal contrasted to the advantages of ease as well as even more exact outcomes, this approach is no longer complimentary. You desire to carry out the complimentary criminal document pascocountyarrests check and also you do not desire to invest a solitary dollar on your lookup; is this truly feasible?

    ReplyDelete
  36. Quick Cure in Eradicating Herpes Simplex Virus by Doctor ELINFOH. I recommend doctor Elinfoh herbal medicine to anyone battling Herpes Infection...I am so happy to be finally cured of herpes simplex virus  by herbalist Elinfoh,Ever since I was diagnosed with the virus on June 24, 2018. I have been using medical drugs to suppress it, I later realized it was causing more damage to my health than the virus,I opted for herbs and got to find out about the great work herbalist Doctor Elinfoh has been doing with his herbal remedy. I contacted him and he promised to help me get rid of it,after 18 days I received a package from the UPS delivery service and it was from the doctor who sent me the herbal treatment in Dallas,Texas USA, And he instructed me on how I was going to take it for the next 3 weeks . After completing the dosage he told me to go for a medical checkup and when I did the doctors conducted some tests on me but they were stunned that my status was now negative,they conducted several tests on me to be sure. I even went to Medical City Dallas Hospital, where they conducted another test and it was still negative. I am so grateful to you herbal doctor Elinfoh , God will continue to prosper you,I pray the world gets to know about you and your herbal exploits. He also cures any other Human diseases.  Reach him on his email drelinfohherbalhome@gmail.com   PLEASE SHARE , SO THE WORLD CAN GET TO KNOW ABOUT HIM.DR ELINFOH HAS THE BEST HERBAL REMEDY FOR HERPES SIMPLEX VIRUS 1&2.

    ReplyDelete
  37. I was diagnosed as HEPATITIS B carrier in 2013 with fibrosis of the
    liver already present. I started on antiviral medications which
    reduced the viral load initially. After a couple of years the virus
    became resistant. I started on HEPATITIS B Herbal treatment from
    ULTIMATE LIFE CLINIC (www.ultimatelifeclinic.com) in March, 2020. Their
    treatment totally reversed the virus. I did another blood test after
    the 6 months long treatment and tested negative to the virus. Amazing
    treatment! This treatment is a breakthrough for all HBV carriers.

    ReplyDelete
  38. DR EMU WHO HELP PEOPLE IN ANY TYPE OF LOTTERY NUMBERS
    It is a very hard situation when playing the lottery and never won, or keep winning low fund not up to 100 bucks, i have been a victim of such a tough life, the biggest fund i have ever won was 100 bucks, and i have been playing lottery for almost 12 years now, things suddenly change the moment i came across a secret online, a testimony of a spell caster called dr emu, who help people in any type of lottery numbers, i was not easily convinced, but i decided to give try, now i am a proud lottery winner with the help of dr emu, i won $1,000.0000.00 and i am making this known to every one out there who have been trying all day to win the lottery, believe me this is the only way to win the lottery.

    Dr Emu can also help you fix this issues

    (1)Ex back.
    (2)Herbal cure & Spiritual healing.
    (3)You want to be promoted in your office.
    (4)Pregnancy spell.
    (5)Win a court case.

    Contact him on email Emutemple@gmail.com
    What’s app +2347012841542
    Website Https://emutemple.wordpress.com/
    Facebook page Https://web.facebook.com/Emu-Temple-104891335203341

    ReplyDelete
  39. Good luck to anyone reading this true life story of mine, I Was Diagnosed With type 2 Herpes Virus Last year, And I Was Looking For Solution To Be Cured Luckily I Saw Testimonies On How Dr OYAGU Cure Herpes Virus I Decided To Contact Dr OYAGU I Contacted Him He Prepared A Herbal Medicine Portion And Sent It To Me, I Started The Herbal Medicine For My Health. He Gave Me Step By Step Instructions On How To Apply It, When I Applied It As Instructed, I Was Cured Of This Deadly Herpes Within 2 weeks, I Am Now Herpes Negative. My Brother And Sister I No That There Are So Many People That Have The Same Herpes Virus Please contact Dr OYAGU To Help You Too, And Help Me To Thank Dr OYAGU For Cure Me, I’m Cured By Dr. OYAGU Herbal Medicine, His Contact Email:oyaguherbalhome@gmail.com or visit his website https://oyaguspellcaster.wixsite.com/oyaguherbalhome Or Cell Whatsapp Number +2348101755322 thank you

    ReplyDelete